×

one step method meaning in Chinese

单步法

Examples

  1. Objective : to clone and sequence the cdna encoding metalloproteinase from the venom of agkistrodon acutus from northen mountain area of guangxi province . methods : one step method was used to extract total rna from the venom of agkistrodon acutus found in northern mountain area of guangxi province . different kinds of cdna encoding metalloproteinase were amplified by one step method ( rt - pcr and pcr reactions occurred in the same tube ) using different primers
    方法:从桂北五步蛇毒腺中抽提总rna ,利用不同的引物,采用一步法( rt - pcr和pcr在同一管内进行)扩增出不同的dna条带,利用平端连接的方法将pcr扩增产物克隆至pgem - teasy载体,转化大肠杆菌jm109 ,挑选白色菌落提取质粒,用pcr对其进行鉴定,直接利用纯化pcr产物或提取阳性菌落质粒进行测序。

Related Words

  1. steps
  2. step
  3. stepping
  4. stepped
  5. step for step
  6. step by step
  7. step one
  8. one step
  9. one step growth curve
  10. one step growth experiment
  11. one step method kit
  12. one step operation
PC Version

Copyright © 2018 WordTech Co.